Description
Description
MW = 14.4 kDa calculated. Human, recombinant, Alpha-synuclein (α-Synuclein) cloned from human cDNA, expressed in E. coli. The protein consists of the full length Alpha-synuclein (residues 1-140, UniProtKB accession P37840).
Sequence
10 20 30 40 50
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH
60 70 80 90 100
GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL
110 120 130 140
GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight of about 14.4 kDa.
Supplied as
0.5 mg/mL solution in sodium phosphate 20 mM pH 6.5, NaCl 200 mM, EDTA 0.5 mM, Complete Protease Inhibitor Cocktail EDTA-free (Roche). The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 5960 M-1cm-1 calculated).
Storage
-80°C. The protein is stable at 4°C for at least 1 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C. Avoid repeated freeze/thaw cycles.