Description
Description
MW = 23.2 kDa calculated. Hemopexin-Like domain of MMP-12 (Macrophage metalloelastase, Matrix metalloproteinase 12), cloned from human cDNA, expressed in E. coli. The protein consists of the hemopexin-like domain of human MMP-12 (residues 278-470, UniProtKB accession P39900 ).
Sequence
280 290 300 310 320
M-EPA LCDPNLSFDA VTTVGNKIFF FKDRFFWLKV SERPKTSVNL
330 340 350 360 370
ISSLWPTLPS GIEAAYEIEA RNQVFLFKDD KYWLISNLRP EPNYPKSIHS
380 390 400 410 420
FGFPNFVKKI DAAVFNPRFY RTYFFVDNQY WRYDERRQMM DPGYPKLITK
430 440 450 460 470
NFQGIGPKID AVFYSKNKYY YFFQGSNQFE YDFLLQRITK TLKSNSWFGC
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 18.4 and 25.0 kDa.
Supplied as
0.1 mg/mL solution in Tris 20 mM pH 7.2, CaCl2 10 mM, ZnCl2 0.1 mM, NaCl 150 mM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 46995 M-1cm-1 calculated).
Storage
-80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C.
Avoid repeated freeze/thaw cycles.